Research Article

Oxidative polymerization and partial dechlorination of 2,4,6-trichlorophenol by a mixture of peroxidase isozymes from Vaccinium myrtillus

  1. Silvia Rita Stazi1,
  2. Pedro Fevereiro2,
  3. Giovanni Giovannozzi Sermanni1,
  4. Raquel Aires Barros3,*

Article first published online: 23 JAN 2001

DOI: 10.1002/jctb.362

Issue

Journal of Chemical Technology and Biotechnology

Journal of Chemical Technology and Biotechnology

Volume 76, Issue 2, pages 210–214, February 2001

Additional Information

How to Cite

Stazi, S. R., Fevereiro, P., Sermanni, G. G. and Barros, R. A. (2001), Oxidative polymerization and partial dechlorination of 2,4,6-trichlorophenol by a mixture of peroxidase isozymes from Vaccinium myrtillus. Journal of Chemical Technology and Biotechnology, 76: 210–214. doi: 10.1002/jctb.362

Author Information

  1. 1

    Dipartimento di Agrobiologia & Agrochimica, Universita della Tuscia, V San Camillo de Lellis snc 01100 Viterbo, Italy

  2. 2

    Laboratorio de Biotecnologia de Plantas, ITQB, Quinta do Marques, 2780 Oeiras, Portugal

  3. 3

    Laboratorio de Engenharia Bioquimica, CEBQ, Instituto Superior Tecnico, Av Roviscos Pais 1000, Lisbon, Portugal

*Laboratorio de Engenharia Bioquimica, CEBQ, Instituto Superior Tecnico, Av Roviscos Pais 1000, Lisbon, Portugal

Publication History

  1. Issue published online: 23 JAN 2001
  2. Article first published online: 23 JAN 2001
  3. Manuscript Accepted: 10 OCT 2000
  4. Manuscript Revised: 27 SEP 2000
  5. Manuscript Received: 1 JUN 2000

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Keywords:

  • chlorophenols;
  • removal;
  • peroxidase;
  • dechlorination;
  • polyethylene glycol;
  • dehalogenation

Abstract

Plant peroxidases (EC 1.11.1.7) catalyze the oxidation of phenolic pollutants in the presence of hydrogen peroxide. In the present study, extracellular peroxidases from Vaccinium myrtillus cell suspension cultures (VMP) were evaluated for their ability to polymerize 2,4,6-trichlorophenol (TCP), a ubiquitous environmental contaminant. The effect of pH, temperature, reaction time, enzyme amount and initial pollutant concentration on the treatment efficiency was investigated in order to optimize the reaction conditions for TCP removal. An appreciable removal efficiency and a partial dehalogenation of TCP was observed over a wide range of initial pollutant concentrations (0.1–20 mmol dm−3) and reaction conditions suggesting that VMP could be useful for potential decontamination applications. The use of polyethylene glycol in the reaction mixture allowed a reduction of the catalyst requirements needed to obtain well defined extents of TCP removal.

© 2001 Society of Chemical Industry

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