Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery
Version of Record online: 16 APR 2012
© 2012 Society of Chemical Industry
Journal of Chemical Technology and Biotechnology
Volume 88, Issue 2, pages 237–246, February 2013
How to Cite
Marcos, M., García-Cubero, M. T., González-Benito, G., Coca, M., Bolado, S. and Lucas, S. (2013), Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery. J. Chem. Technol. Biotechnol., 88: 237–246. doi: 10.1002/jctb.3820
- Issue online: 22 JAN 2013
- Version of Record online: 16 APR 2012
- Manuscript Accepted: 14 MAR 2012
- Manuscript Revised: 13 MAR 2012
- Manuscript Received: 7 FEB 2012
- enzymatic hydrolysis;
- wheat straw
BACKGROUND: The enzymatic hydrolysis of steam-exploded wheat straw using commercial enzyme complexes has been studied. A cellulase enzyme complex (Accellerase 1500), along with specific xylanase complements (Accellerase-XC and Accellerase-XY) provided by Genencor, have been used to enhance glucose and xylose recovery.
A systematic study with response surface methodology (RSM) was used to check the effect of the operating conditions: pH (4–5), temperature (50–60 °C) and enzyme/substrate ratio (0.1–0.5 mL gcellulose−1) on the enzymatic hydrolysis with Acellerase 1500 to maximize the sugar yield. Xylanases were used as complements to increase the release of xylose.
RESULT: Statistical results from ANOVA analysis demonstrated that the enzymatic hydrolysis was clearly improved by temperature and enzyme/substrate ratio. The optimum conditions for higher glucose and xylose releases were obtained with the higher enzyme dosage ratio (0.5 mL g−1cellulose), 50 °C and pH 4.
CONCLUSION: Model validation at optimum operating conditions showed good agreement between the experimental results and the predicted responses for a confidence level of 95%.
The use of the xylanase complements, Accellerase-XY (accessory xylanase enzyme complex) and Accellerase-XC (accessory xylanase/cellulase enzyme complex), increases the conversion of hemicellulose. Accellerase-XC supplementation was more effective, obtaining an increase in yields of glucose and xylose of 11.8% and 23.6%, respectively, using a dosage of 0.125 mL g−1cellulose. © 2012 Society of Chemical Industry