Comparative physicochemical analysis of suspended and immobilized cultivation of Chlorella sp.

Authors

  • Xianhai Zeng,

    1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 China
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  • Michael K. Danquah,

    Corresponding author
    1. Department of Chemical Engineering, Monash University, Clayton 3800 VIC, Australia
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  • Ronald Halim,

    1. Department of Chemical Engineering, Monash University, Clayton 3800 VIC, Australia
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  • Shuyu Yang,

    1. Department of Endocrinology, Xiamen Diabetes Institute, the First Affiliated Hospital of Xiamen University, Xiamen, 361001 China
    2. The Medical College, Xiamen University, Xiamen 361005 China
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  • Xiao Dong Chen,

    1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 China
    2. Department of Chemical Engineering, Monash University, Clayton 3800 VIC, Australia
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  • Yinghua Lu

    Corresponding author
    1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 China
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Yinghua Lu, Department of Chemical and Biochemical Engineering, College of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 China. E-mail: ylu@xmu.edu.cn

Michael K. Danquah, Department of Chemical Engineering, Monash University, Clayton 3800 VIC, Australia. E-mail: Michael.Danquah@monash.edu; mkdanquah@gmail.com

Abstract

BACKGROUND: Immobilized cultivation of microorganisms is gaining interest in the microalgae industry. In this study, a novel microalgae polymeric carrier, sodium cellulose sulphate/poly-dimethyl-diallyl-ammonium chloride (NaCS-PDMDAAC) capsule system, was employed to immobilize Chlorella sp. Microalgae cultivation was performed under immobilized and suspended conditions, and the resulting cells were characterized biochemically and physically.

RESULTS: Experimental results indicated that microalgae cells cultivated under NaCS-PDMDAAC immobilized conditions have a robust morphology, a smaller average cell size of 2.92 µm and a higher Zeta potential of − 17.10 mV than the suspended cells with a cell size of 6.81 µm and Zeta potential of − 32.90 mV. The immobilized microalgae cells displayed a total lipid content of 14.85%, and a chlorophyll content of 3.36%, slightly higher than those obtained from the suspended cells.

CONCLUSION: These differences are critical to further explore the impact of immobilized microalgae cultivation systems for specific biotechnological applications. © 2012 Society of Chemical Industry

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