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Liquefied petroleum gas as solvent medium for the treatment of immobilized inulinases

Authors

  • Marceli Fernandes Silva,

    1. Department of Food Engineering, URI—Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil
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  • Simone Maria Golunski,

    1. Department of Food Engineering, URI—Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil
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  • Diane Rigo,

    1. Department of Food Engineering, URI—Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil
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  • Vinícius Mossi,

    1. Department of Food Engineering, URI—Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil
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  • Marco Di Luccio,

    1. Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitário, Trindade, Caixa Postal 476, Florianópolis, SC, 88040-900, Brazil
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  • Marcio A. Mazutti,

    1. Department of Chemical Engineering - Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil
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  • Sibele B.C. Pergher,

    1. Department of Chemistry—Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Lagoa Nova, Natal, RN, 59078-970, Brazil
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  • Débora Oliveira,

    1. Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitário, Trindade, Caixa Postal 476, Florianópolis, SC, 88040-900, Brazil
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  • J. Vladimir Oliveira,

    1. Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitário, Trindade, Caixa Postal 476, Florianópolis, SC, 88040-900, Brazil
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  • Helen Treichel

    Corresponding author
    1. Chemistry and Food School, Federal University of Rio Grande - FURG P.O Box 474, CEP: 96201900, Rio Grande - RS, Brazil
    2. Federal University of Fronteira Sul - Campus de Erechim, Av. Dom João Hoffmann, 313, 99700-000, Erechim, RS, Brazil
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Helen Treichel, Chemistry and Food School, Federal University of Rio Grande - FURG P.O Box 474, CEP: 96201900, Rio Grande - RS, Brazil. E-mail: helentreichel@gmail.com

Abstract

BACKGROUND: The main goal of this work is to assess the influence of pressurized liquefied petroleum gas (LPG) treatment on the enzymatic activity of immobilized inulinases. The effects of process variables were evaluated through 23 experimental design.

RESULTS: Inulinase from Klyveromyces marxianus NRRL Y-7571 presented an increase of 163% in residual activity using LPG at 30 bar during 1 h exposure using a depressurization rate of 20 bar min−1. For Aspergillus niger commercial inulinase, an increment of 129% in residual activity was observed at 30 bar for 1 h treatment at the highest depressurization rate (20 bar min−1). Enzymatic activities changed significantly depending on the enzyme source and the experimental treatment conditions investigated, such as exposure time, depressurization rate and pressure.

CONCLUSION: Hence, compressed LPG appears to be a promising technique with practical relevance as a preparation step to improve enzyme activity, thus helping the development of new biotransformation processes. © 2012 Society of Chemical Industry

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