11α hydroxylation of 16α, 17-epoxyprogesterone in biphasic ionic liquid/water system by Aspergillus ochraceus

Authors

  • Shuhong Mao,

    1. Key Laboratory of Fermentation Microbiology, Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457, China
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  • Boyuan Hua,

    1. Key Laboratory of Fermentation Microbiology, Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457, China
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  • Na Wang,

    1. National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science & Technology, Tianjin 300457, China.
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  • Xiaojie HU,

    1. Key Laboratory of Fermentation Microbiology, Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457, China
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  • Zhijiang Ge,

    1. Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
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  • Yanqing Li,

    1. Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
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  • Sha Liu,

    1. Key Laboratory of Fermentation Microbiology, Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457, China
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  • Fuping Lu

    Corresponding author
    1. Key Laboratory of Fermentation Microbiology, Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457, China
    2. National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science & Technology, Tianjin 300457, China.
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Fuping Lu, Industrial Microbiology Laboratory, College of Biotechnology, Tianjin University of Science & Technology, No. 29, 13 Main Street, Tianjin Economic and Technological Development Zone, Tianjin 300457, China. E-mail: lfp@tust.edu.cn

Abstract

BACKGROUND: The improved efficiency of steroid biotransformation using the biphasic system is generally attributed to the positive effect on the solubility of substrate in aqueous media. A promising alternative for the application of organic solvents in biphasic systems is the use of ionic liquids (ILs). This study aims to investigate the applicability of the biphasic ILs/water system for 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by Aspergillus ochraceus.

RESULTS: Of the seven ILs tested, [C3mim][PF6] exhibited the best biocompatibility, with markedly improved biotransformation efficiency. In the [C3mim][PF6]-based biphasic system, substrate conversion reached 90% under the condition in which buffer pH, volume ratio of buffer to ILs, cell concentration, and substrate concentration were 4.8, 10/1, 165 g L−1 and 20 g L−1, respectively. This is more efficient than that of the monophasic aqueous system. The effects of the cations and anions of these ILs on the 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by A. ochraceus is also discussed.

CONCLUSION: The above results showed that IL/water biphasic system improved the efficiency of 11α hydroxylation of 16α, 17-epoxyprogesterone (HEP) by A. ochraceus, thus suggesting the potential industrial application of ILs-based biphasic systems for steroid biotransformation. © 2012 Society of Chemical Industry

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