Cell immobilization technique for biotrickle filtering of isopropyl alcohol waste vapor generated by high-technology industries

Authors

  • Shen-Long Tsai,

    1. Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA
    Search for more papers by this author
  • Chi-Wen Lin,

    Corresponding author
    • Department of Safety, Health and Environmental Engineering, National Yunlin University of Science and Technology, 123 University Road, Section 3, Douliou, Yunlin 64002, Taiwan
    Search for more papers by this author
  • Chih-Hung Wu,

    1. Graduate School of Engineering Science and Technology, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan
    Search for more papers by this author
  • Baoping Xin

    1. Department of Environmental Engineering and Energy, School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, P.R. China
    Search for more papers by this author

Correspondence to: Chi-Wen Lin, Department of Safety, Health and Environmental Engineering, National Yunlin University of Science and Technology, 123 University Rd. Sec. 3, Douliou, Yunlin 64002, Taiwan. E-mail: Linwen@yuntech.edu.tw

Abstract

BACKGROUND: A bench-scale biotrickling filter coupled with Pseudomonas citronellolis YAIP521-immobilized polyvinyl alcohol (PVA)/alginate beads was developed for kinetic analysis of microbial removal of isopropyl alcohol (IPA), an organic solvent widely used for fabricating wafers and printed circuit boards.

RESULTS: Response surface methodology (RSM) showed that the optimal ratio of PVA to alginate was 7.5 g to 0.8 g. More than 95% of IPA removal could be achieved at an inlet concentration of 220 ± 34 ppm (w/w) under short residency time. System stability decreased under high IPA concentration and intermittent shock-loading conditions but increased when using cell-immobilized beads because the buffer effect limited the adverse impacts of high IPA concentrations on microorganisms, and the system gradually stabilized with IPA removal efficiency as high as 95%. Nevertheless, qPCR indicated that intermittent shock-loading decreased the biomass in the beads.

CONCLUSION: The experimental results showed that the biotrickling filter system developed effectively diminishes the inhibitory effects of elevated IPA concentration on microbial growth, thereby solving the problem of high IPA loading often encountered in the electronic high-tech industries. The design of the system along with the population dynamics and reaction kinetics provide superior information to ensure the success of the biotrickling filter system. © 2012 Society of Chemical Industry

Ancillary