Enzymatic saccharification of acid–alkali pretreated sugarcane bagasse using commercial enzyme preparations
Article first published online: 24 DEC 2012
© 2012 Society of Chemical Industry
Journal of Chemical Technology and Biotechnology
Volume 88, Issue 7, pages 1266–1272, July 2013
How to Cite
Giese, E. C., Pierozzi, M., Dussán, K. J., Chandel, A. K. and da Silva, S. S. (2013), Enzymatic saccharification of acid–alkali pretreated sugarcane bagasse using commercial enzyme preparations. J. Chem. Technol. Biotechnol., 88: 1266–1272. doi: 10.1002/jctb.3968
- Issue published online: 6 JUN 2013
- Article first published online: 24 DEC 2012
- Manuscript Accepted: 26 SEP 2012
- Manuscript Revised: 24 SEP 2012
- Manuscript Received: 5 AUG 2012
- sugarcane bagasse;
- acid–alkali pretreatment;
- enzymatic hydrolysis;
- cellulase AF28918;
- scanning electron microscopy
The bioconversion of lignocellulosic biomass to second generation ethanol has the potential to replace some fossil fuels. Sugarcane bagasse (SB) is a readily available feedstock for ethanol production. The aim of this work was to study the enzymatic saccharification of consecutively acid–alkali pretreated SB using different commercial enzyme preparations.
Under the most favourable enzymatic hydrolysis conditions, a maximum sugar recovery of 0.89 g of reducing sugars g-1 acid–alkali pretreated SB was obtained. Scanning electron microscopy (SEM) revealed the structural disruption in the SB morphology after acid–alkali pretreatment followed by enzymatic hydrolysis.
These results improve research strategies and show the susceptibility of SB under different enzymatic actions. This is thought to be the first report on utilization of cellulase AF28918 for SB saccharification into fermentable sugars.