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jctb3975-sup-0001-FigureS1.docWord document1505KCLSM images of an activated sludge floc stained without sectioning for EPS and bacteria visualization: a) maximum intensity projections of the confocal slices of the sample stained with three different dyes (red–lipids, green–esterase producing bacteria, blue–α-polysaccharides) and the combined image; b) transmission image of the studied specimen; c) 3-D reconstruction of the floc.
jctb3975-sup-0002-FigureS2.docWord document206Khistograms showing the distribution of lipids,esterase-producing bacteria and α-polysaccharides within the sample presented on Fig S1.
jctb3975-sup-0003-FigureS3.docWord document212KCLSM images of antibody and dye stained samples without sectioning for visualization of alkaline phosphatase (ab60317, red), α-polysaccharides (ConA488, green), total cells (Syto, blue) and the combined image
jctb3975-sup-0004-FigureS4.docWord document271KCLSM images of antibody and dye stained samples without sectioning for visualization of Trypsin (ab60317, red), α-polysaccharides (ConA488, green), total cells (Syto, blue) and the combined image
jctb3975-sup-0005-FigureS5.docWord document226KCLSM images of antibody and dye stained samples without sectioning for control purposes:Red- antibody staining specificity control (secondary antibody alone); green – α-polysaccharides (ConA488), blue – total cells (Syto).
jctb3975-sup-0006-FigureS6.docWord document305KCLSM images of a whole activated sludge sample stained for the simultaneous visualization of alkaline phosphatase (ab60317, red) and β-glycosidaseenzymes (ab60313, green), for total cells (Syto63, blue) and the combined image of the stained sample.
jctb3975-sup-0007-FigureS7.docWord document237KCLSM images of a whole activated sludge sample stained for the simultaneous visualization of trypsin (ab60317, red) and β-glycosidase enzymes (ab60313, green), total cells (Syto63, blue) and the merged image of the stained sample.
jctb3975-sup-0008-FigureS8.docWord document437KThe CLSM images of antibody and dye stained 5 µm thick sectioned sample for the visualization of β-polysaccharides (calcofluor white, blue), total cells (Syto 63, yellow), alkaline phosphatase (ab60317, red) and β-glycosidase (ab60313, green)
jctb3975-sup-0009-FigureS9.docWord document416KThe CLSM images of antibody and dye stained 5 µm thick sectioned sample for the visualization of β-polysaccharides (calcofluor white, blue), total cells (Syto 63, yellow), trypsin (ab60317, red) and β-glycosidase (ab60313, green)
jctb3975-sup-0010-FigureS10.docWord document23K3D reconstruction of an activated sludge floc stained and imaged without sectioning. Link to the video: http://www.youtube.com/watch?v=_ywezMcMzIc

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