A novel process for the recovery of superoxide dismutase from yeast exploiting electroextraction coupled to direct sorption

Authors

  • Jesús Simental-Martínez,

    1. Downstream Bioprocessing Laboratory, Jacobs University, Bremen, Germany
    2. Centro de Biotecnología-FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico
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  • Rami Reddy Vennapusa,

    1. Downstream Bioprocessing Laboratory, Jacobs University, Bremen, Germany
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  • Jorge Benavides,

    1. Centro de Biotecnología-FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico
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  • Valentina Ganeva,

    1. Faculty of Biology, University of Sofia, Sofia, Bulgaria
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  • Bojidar Galutzov,

    1. Faculty of Biology, University of Sofia, Sofia, Bulgaria
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  • Marco Rito-Palomares,

    1. Centro de Biotecnología-FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico
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  • Marcelo Fernández-Lahore

    Corresponding author
    1. Centro de Biotecnología-FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico
    • Downstream Bioprocessing Laboratory, Jacobs University, Bremen, Germany
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  • Part of this work was presented at the 16th International Conference on BioPartitioning and Purification 2011, held at Puerto Vallarta, Jalisco, Mexico in September 2011.

Correspondence to: Marcelo Fernández-Lahore, Jacobs University, Campus Ring 1, D-28759 Bremen, Germany. E-mail: m.fernandez-lahore@jacobs-university.de

Abstract

Background

This work presents a novel approach for the recovery and purification of superoxide dismutase (SOD; EC 1.15.1.1) produced in the yeast Kluyveromyces marxianus var. bulgaricus (NBIMCC 1984). Electroextraction was evaluated as an alternative to bead milling for SOD release. Seven anion exchange resins were studied in order to optimize SOD purification using ion exchange chromatography.

Results

Electroextraction produced an enzyme solution with a specific activity 13 times higher than with mechanical extraction, demonstrating the potential of this novel technique for the selective liberation of SOD. Regarding the anion exchange resins that were studied, Amberlite™ IRA-96 and Q Sepharose™ XL were chosen as the best options for purifying SOD using chromatography in negative adsorption mode. The process developed, involving electroextraction, enzyme concentration and negative anion exchange chromatography, rendered a highly active and pure SOD preparation (specific activity: 2056 U mg−1 and 66-fold purification) with an overall recovery yield of 84%.

Conclusion

The results presented herein demonstrate the potential of the process developed for the recovery and purification of SOD from the yeast Kluyveromyces marxianus var. bulgaricus (NBIMCC 1984). The preparation obtained could be used in the food industry as an antioxidant agent. © 2013 Society of Chemical Industry

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