Bioreactors are widely used in the chemical, food and medical industries. However, enzyme immobilization in the bioreactor often results in the loss of activity and stability to varying degrees. To resolve the problem, a new functional conducting polymer monomer, 2,5-di(2-thienyl)-1H-pyrrole-1-(p-phenylacetic acid) (DPP), was designed, synthesized and electrodeposited on copper net to form ultrathin poly(DPP) film containing free carbonyl groups. Then, pseudomonas cepacia lipase was covalently coated on the film with 1-ethyl-3-(3-dimethyl-aminopropyl)-carbodiimide /N-hydroxy-succinimide as activator, and 1,3-di(isobutyl)-imidazolium hexafluorophosphate ionic liquid was finally coated on the surface of the film. In this study, an as-prepared bioreactor was employed for the resolution of (R,S)-1-phenylethanol to investigate catalytic characterization of the enzyme immoblized on the bioreactor.