Research Article

Production of fructose and ethanol from media with high sucrose concentrations by a mutant of Saccharomyces cerevisiae

  1. Hasan Atiyeh,
  2. Zdravko Duvnjak*

Article first published online: 16 AUG 2001

DOI: 10.1002/jctb.474

Issue

Journal of Chemical Technology and Biotechnology

Journal of Chemical Technology and Biotechnology

Volume 76, Issue 10, pages 1017–1022, October 2001

Additional Information

How to Cite

Atiyeh, H. and Duvnjak, Z. (2001), Production of fructose and ethanol from media with high sucrose concentrations by a mutant of Saccharomyces cerevisiae. Journal of Chemical Technology and Biotechnology, 76: 1017–1022. doi: 10.1002/jctb.474

Author Information

  1. Department of Chemical Engineering, University of Ottawa, Ottawa, Canada, K1N 6N5

*Department of Chemical Engineering, University of Ottawa, Ottawa, Canada, K1N 6N5

Publication History

  1. Issue published online: 16 AUG 2001
  2. Article first published online: 16 AUG 2001
  3. Manuscript Accepted: 15 MAY 2001
  4. Manuscript Revised: 22 MAR 2001
  5. Manuscript Received: 3 JAN 2001

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Keywords:

  • Saccharomyces cerevisiae;
  • fructose;
  • sucrose;
  • ethanol;
  • fructo-oligosaccharides;
  • glycerol

Abstract

The production of enriched fructose syrups and ethanol from a synthetic medium with high sucrose concentrations was studied in a batch process using Saccharomyces cerevisiae ATCC 36858. The results showed that the fructose yield was above 92% of theoretical values in synthetic media with sucrose concentrations between 180 g dm−3 and 726 g dm−3. Ethanol yield was about 82% in media with sucrose concentrations up to 451 g dm−3. A product containing 178 g dm−3 fructose, which represents 97% of the total sugar content, and 79 g dm−3 ethanol was obtained using a medium with 360 g dm−3 sucrose. The fructose fraction in the carbohydrates content in the produced syrups decreased with increases in the initial sucrose concentration. In a medium with initial sucrose concentration of 574 g dm−3, the fructose content in the produced broth was 59% of the total carbohydrates. Glycerol and fructo-oligosaccharides were also produced in this process. The produced fructo-oligosaccharides started to be consumed when the concentration of sucrose in the media was less than 30% of its initial value. Complete hydrolysis of these sugars was noticed in media with sucrose concentrations below 451 g dm−3. These findings will be useful in the production of ethanol and high fructose syrups using sucrose-based raw materials with high concentrations of this carbohydrate.

© 2001 Society of Chemical Industry

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