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Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy

Authors

  • Li Zhang,

    1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China
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  • Hong Shen,

    1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China
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  • Yuanxing Zhang

    Corresponding author
    1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China
    • State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China
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Abstract

The achievement of high cell density and monoclonal antibody concentration depends on understanding cell metabolism and the control of cell culture. A model of cell metabolism and monoclonal antibody production in hybridoma cell culture is presented. Fed-batch culture in serum-free medium was conducted in a bioreactor with an optimized feeding strategy according to the model. In the process, nutrient concentrations, especially glucose and glutamine, were maintained at relatively constant and low levels. The formation of toxic byproducts, such as ammonia and lactate, was greatly reduced in comparison with that in non-optimized batch culture. The concentration of monoclonal antibody reached 350 mg dm−3, which was seven times greater than in non-optimized batch culture. Copyright © 2003 Society of Chemical Industry

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