Topical Paper

Progenitor cells of the olfactory receptor neuron lineage

  1. Anne L. Calof*,
  2. Alexandre Bonnin,
  3. Candice Crocker,
  4. Shimako Kawauchi,
  5. Richard C. Murray,
  6. Jianyong Shou†,
  7. Hsiao-Huei Wu

Article first published online: 23 JUL 2002

DOI: 10.1002/jemt.10147

Issue

Microscopy Research and Technique

Microscopy Research and Technique

Special Issue: Structure and Function of Olfactory Neuroepithelium

Volume 58, Issue 3, pages 176–188, 1 August 2002

Additional Information

How to Cite

Calof, A. L., Bonnin, A., Crocker, C., Kawauchi, S., Murray, R. C., Shou, J. and Wu, H.-H. (2002), Progenitor cells of the olfactory receptor neuron lineage. Microscopy Research and Technique, 58: 176–188. doi: 10.1002/jemt.10147

Author Information

  1. Department of Anatomy and Neurobiology and the Developmental Biology Center, University of California, Irvine, College of Medicine, Irvine, California 92697-1275

  1. †

    Lilly Research Labs-Functional Genomics, Eli Lilly and Company, Indianapolis, IN 46285

*Dept. of Anatomy and Neurobiology, 364 Med Surge II, University of California, Irvine, College of Medicine, Irvine, CA 92697-1275

Publication History

  1. Issue published online: 23 JUL 2002
  2. Article first published online: 23 JUL 2002
  3. Manuscript Accepted: 20 MAY 2001
  4. Manuscript Received: 26 APR 2001

Funded by

  • National Institutes of Health. Grant Numbers: R01-DC 03583, P01-HD38761
  • March of Dimes Birth Defects Foundation. Grant Number: 1-FY00-660

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Keywords:

  • stem cell;
  • neurogenesis;
  • transcription factors;
  • bone morphogenetic proteins;
  • fibroblast growth factors

Abstract

The olfactory epithelium of the mouse has many properties that make it an ideal system for studying the molecular regulation of neurogenesis. We have used a combination of in vitro and in vivo approaches to identify three distinct stages of neuronal progenitors in the olfactory receptor neuron lineage. The neuronal stem cell, which is ultimately responsible for continual neuron renewal in this system, gives rise to a transit amplifying progenitor identified by its expression of a transcription factor, MASH1. The MASH1-expressing progenitor gives rise to a second transit amplifying progenitor, the Immediate Neuronal Precursor, which is distinct from the stem cell and MASH1-expressing progenitor, and gives rise quantitatively to olfactory receptor neurons. Regulation of progenitor cell proliferation and differentiation occurs at each of these three cell stages, and growth factors of the fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) families appear to play particularly important roles in these processes. Analyses of the actions of FGFs and BMPs reveal that negative signaling plays at least as important a role as positive signaling in the regulation of olfactory neurogenesis. Microsc. Res. Tech. 58:176–188, 2002. © 2002 Wiley-Liss, Inc.

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