Notes on theory and experimental conditions behind two-photon excitation microscopy
Article first published online: 10 DEC 2003
Copyright © 2003 Wiley-Liss, Inc.
Microscopy Research and Technique
Special Issue: Two-Photon Microscopy—Part II
Volume 63, Issue 1, pages 12–17, 1 January 2004
How to Cite
Esposito, A., Federici, F., Usai, C., Cannone, F., Chirico, G., Collini, M. and Diaspro, A. (2004), Notes on theory and experimental conditions behind two-photon excitation microscopy. Microsc. Res. Tech., 63: 12–17. doi: 10.1002/jemt.10425
- Issue published online: 10 DEC 2003
- Article first published online: 10 DEC 2003
- Manuscript Accepted: 29 SEP 2003
- Manuscript Received: 10 AUG 2003
- two-photon excitation;
- fluorescence emission;
- optical sectioning effect
This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission. Microsc. Res. Tech. 63:12–17, 2004. © 2003 Wiley-Liss, Inc.