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Keywords:

  • two-photon excitation;
  • fluorescence emission;
  • optical sectioning effect

Abstract

This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission. Microsc. Res. Tech. 63:12–17, 2004. © 2003 Wiley-Liss, Inc.