Notes on theory and experimental conditions behind two-photon excitation microscopy

Authors

  • Alessandro Esposito,

    1. Laboratory for Advanced Microscopy, Department of Physics, University of Genoa, 16146 Genoa, Italy
    2. Istituto Nazionale per la Fisica della Materia, Department of Physics, University of Genoa, 16146 Genoa, Italy
    3. CELL BIOPHYSICS Group, European Neuroscience Institute, Göttingen 33 37073, Germany
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  • Federico Federici,

    1. Laboratory for Advanced Microscopy, Department of Physics, University of Genoa, 16146 Genoa, Italy
    2. Istituto Nazionale per la Fisica della Materia, Department of Physics, University of Genoa, 16146 Genoa, Italy
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  • Cesare Usai,

    1. Laboratory for Advanced Microscopy, Department of Physics, University of Genoa, 16146 Genoa, Italy
    2. National Research Council, Institute of Biophysics, 16149 Genoa, Italy
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  • Fabio Cannone,

    1. Laboratory for Advanced Bio-Spectroscopy and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
    2. INFM and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
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  • Giuseppe Chirico,

    1. Laboratory for Advanced Bio-Spectroscopy and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
    2. INFM and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
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  • Maddalena Collini,

    1. Laboratory for Advanced Bio-Spectroscopy and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
    2. INFM and Department of Physics, University of Milano Bicocca, 20126, Milano, Italy
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  • Alberto Diaspro

    Corresponding author
    1. Laboratory for Advanced Microscopy, Department of Physics, University of Genoa, 16146 Genoa, Italy
    2. Istituto Nazionale per la Fisica della Materia, Department of Physics, University of Genoa, 16146 Genoa, Italy
    • LAMBS, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy
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Abstract

This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission. Microsc. Res. Tech. 63:12–17, 2004. © 2003 Wiley-Liss, Inc.

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