Immunohistochemical characterization of adenosine receptors in rat aorta and tail arteries
Version of Record online: 20 JUN 2008
Copyright © 2008 Wiley-Liss, Inc.
Microscopy Research and Technique
Volume 71, Issue 10, pages 703–709, October 2008
How to Cite
Leal, S., Sá, C., Gonçalves, J., Fresco, P. and Diniz, C. (2008), Immunohistochemical characterization of adenosine receptors in rat aorta and tail arteries. Microsc. Res. Tech., 71: 703–709. doi: 10.1002/jemt.20609
- Issue online: 12 SEP 2008
- Version of Record online: 20 JUN 2008
- Manuscript Accepted: 11 APR 2008
- Manuscript Received: 20 SEP 2007
- FCT. Grant Number: POCI/SAU-FCF/60782/2004
- Fundação Ilídio Pinho
- elastic artery;
- muscular artery;
Adenosine plays an important role in the cardiovascular system, activating adenosine A1, A2A, A2B, and A3 receptors, and regulating blood flow either by acting directly on vascular cells or indirectly because of its effects on the central or peripheral nervous systems. The aim of the present study was to investigate whether the pattern of distribution of adenosine receptor subtypes is different on elastic and muscular, using abdominal aorta and tail arteries as models. Immunohistochemistry using anti-A1, anti-A2A, anti-A2B, and anti-A3 receptor antibodies was performed on perfused-fixed/paraffin-embedded arteries from Wistar rats. 3,3′-Diaminobenzidine tetrahydrochloride (DAB; activated by hydrogen peroxide) staining revealed significant differences in the abundance of A1, A2A, and A3 receptors between abdominal aorta and tail artery and allowed the identification of distinct distribution patterns for A1, A2A, A2B, and A3 receptors in the tunica adventitia, media, and intima of muscular and elastic arteries. Data are compatible with several previous functional reports supporting that different adenosine receptor subtype expression and/or their distribution in the vessel wall may influence their respective contribution to the control of blood flow. Microsc. Res. Tech., 2008. © 2008 Wiley-Liss, Inc.