Contribution to the study of the vasculature of submandibular and sublingual glands and lymph nodes of rats by corrosion cast technique combined with scanning electron microscopy
Article first published online: 24 JUL 2008
Copyright © 2008 Wiley-Liss, Inc.
Microscopy Research and Technique
Volume 71, Issue 10, pages 737–741, October 2008
How to Cite
Rossi-Schneider, T. R., Verli, F. D., Yurgel, L. S., De Souza, M. A. L. and Cherubini, K. (2008), Contribution to the study of the vasculature of submandibular and sublingual glands and lymph nodes of rats by corrosion cast technique combined with scanning electron microscopy. Microsc. Res. Tech., 71: 737–741. doi: 10.1002/jemt.20615
- Issue published online: 12 SEP 2008
- Article first published online: 24 JUL 2008
- Manuscript Accepted: 21 APR 2008
- Manuscript Received: 24 APR 2007
- corrosion casting;
- salivary glands
The study of anatomical structures in their normal state allows the identification of pathological changes that can occur in them. Angiogenesis and the vasculature have been widely studied, mainly because of their association with the development of neoplasms. One of the methods applied for such purposes is the corrosion cast technique, which provides a copy of the vessels with normal as well as pathological structures. The replica of the vasculature provided by this technique allows the three-dimensional analysis of vessels by means of scanning electron microscopy. The aim of the present study was to demonstrate, by means of corrosion casts, the angioarchitecture of the submandibular and sublingual glands and lymph nodes. Scanning electron microscopy showed that the three structures have distinct vascular patterns. The corrosion cast technique can be employed in the study of the angioarchitecture of the submandibular and sublingual glands and lymph nodes, but requires specific precautions. The removal of the structures en bloc and the handling of the replicas with the aid of a stereoscopic magnifier reduce the risk of fractures. Microsc. Res. Tech., 2008. © 2008 Wiley-Liss, Inc.