Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root-peel extract of Millettia pachycarpa
Article first published online: 2 SEP 2008
Copyright © 2008 Wiley-Liss, Inc.
Microscopy Research and Technique
Volume 71, Issue 11, pages 810–815, November 2008
How to Cite
Roy, B., Dasgupta, S. and Tandon, V. (2008), Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root-peel extract of Millettia pachycarpa. Microsc. Res. Tech., 71: 810–815. doi: 10.1002/jemt.20623
- Issue published online: 24 OCT 2008
- Article first published online: 2 SEP 2008
- Manuscript Accepted: 21 JUN 2008
- Manuscript Received: 27 MAY 2008
- G. B. Pant Institute of Himalayan Environment and Development (Ministry of Environment and Forest, Government of India)
- cestode parasite;
- natural product;
Millettia pachycarpa Benth (Leguminosae) has a usage in traditional medicine system practiced among the Lushai tribes of Mizoram, a state in North East India, who customarily consume the aqueous extract of the root peel of the plant to get rid of intestinal worm infections. The crude ethanol, methanol, and acetone fractions of the plant were assayed against Raillietina echinobothrida, the intestinal cestode parasite of domestic fowl, to authenticate the putative anthelmintic efficacy and cestocidal potential in particular of the plants. In vitro exposure of the worm to the extract at a concentration of 25 mg/mL phosphate buffered saline (at 37°C ± 1°C) revealed distortion and disruption of mitochondria, nucleus, nucleolus, nuclear membrane, basal lamina, and tegumental vacuolization in the distal cytoplasm leading to scar formation in the surface. The possible use of the plant as a potential anthelmintic against cestode parasite is discussed. Microsc. Res. Tech., 2008. © 2008 Wiley-Liss, Inc.