The authors wish to dedicate this paper to the memory of Prof. Giuseppe Tomassetti who always encouraged this research field.
X-ray microscopy of plant cells by using LiF crystal as a detector†
Article first published online: 10 SEP 2008
Copyright © 2008 Wiley-Liss, Inc.
Microscopy Research and Technique
Volume 71, Issue 12, pages 839–848, December 2008
How to Cite
Reale, L., Bonfigli, F., Lai, A., Flora, F., Poma, A., Albertano, P., Bellezza, S., Montereali, R. M., Faenov, A., Pikuz, T., Almaviva, S., Vincenti, M. A., Francucci, M., Gaudio, P., Martellucci, S. and Richetta, M. (2008), X-ray microscopy of plant cells by using LiF crystal as a detector. Microsc. Res. Tech., 71: 839–848. doi: 10.1002/jemt.20625
- Issue published online: 18 NOV 2008
- Article first published online: 10 SEP 2008
- Manuscript Revised: 22 APR 2008
- Manuscript Received: 18 JAN 2008
- Consorzio di Ricerca Gran Sasso
- soft X-rays;
- contact microscopy;
A lithium fluoride (LiF) crystal has been utilized as a new soft X-ray detector to image different biological samples at a high spatial resolution. This new type of image detector for X-ray microscopy has many interesting properties: high resolution (nanometer scale), permanent storage of images, the ability to clear the image and reuse the LiF crystal, and high contrast with greater dynamic range. Cells of the unicellular green algae Chlamydomonas dysosmos and Chlorella sorokiniana, and pollen grains of Olea europea have been used as biological materials for imaging. The biological samples were imaged on LiF crystals by using the soft X-ray contact microscopy and contact micro-radiography techniques. The laser plasma soft X-ray source was generated using a Nd:YAG/Glass laser focused on a solid target. The X-ray energy range for image acquisition was in the water-window spectral range for single shot contact microscopy of very thin biological samples (single cells) and around 1 keV for multishots microradiography. The main aim of this article is to highlight the possibility of using a LiF crystal as a detector for the biological imaging using soft X-ray radiation and to demonstrate its ability to visualize the microstructure within living cells. Microsc. Res. Tech., 2008. © 2008 Wiley-Liss, Inc.