Research Article

3D reconstruction of histological sections: Application to mammary gland tissue

  1. Ignacio Arganda-Carreras1,*,
  2. Rodrigo Fernández-González2,
  3. Arrate Muñoz-Barrutia3,
  4. Carlos Ortiz-De-Solorzano3

Article first published online: 15 MAR 2010

DOI: 10.1002/jemt.20829

Issue

Microscopy Research and Technique

Microscopy Research and Technique

Volume 73, Issue 11, pages 1019–1029, October 2010

Additional Information

How to Cite

Arganda-Carreras, I., Fernández-González, R., Muñoz-Barrutia, A. and Ortiz-De-Solorzano, C. (2010), 3D reconstruction of histological sections: Application to mammary gland tissue. Microsc. Res. Tech., 73: 1019–1029. doi: 10.1002/jemt.20829

Author Information

  1. 1

    Biocomputing Unit, National Centre for Biotechnology (CNB-CSIC), Universidad Autónoma de Madrid, 28049 Madrid, Spain

  2. 2

    Developmental Biology Program, Sloan-Kettering Institute, New York, New York

  3. 3

    Cancer Imaging Laboratory, Centre for Applied Medical Research (CIMA), Pamplona, Spain

*Biocomputing Unit, National Centre for Biotechnology (CNB-CSIC), Universidad Autónoma de Madrid, 28049 Madrid, Spain

Publication History

  1. Issue published online: 15 MAR 2010
  2. Article first published online: 15 MAR 2010
  3. Manuscript Accepted: 5 JAN 2010
  4. Manuscript Received: 14 AUG 2009

Funded by

  • Spanish Ministry of Education (MCYT Program). Grant Number: TEC2005-04732
  • Spanish Science and Innovation Ministry. Grant Number: MCIIN PSS-010000-2008-2
  • Marie Curie International Reintegration Grant. Grant Number: MIRG-CT-2005-028342
  • Department of Defense and University of California Breast Cancer Research Programs. Grant Numbers: DAMD17-00-1-0227, DAMD17-00-1-0306, 8WB-0150
  • UTE project CIMA
  • Spanish Ministry of Education (Ramón y Cajal fellowship)

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Keywords:

  • computer-assisted microscopy;
  • assisted histopathology;
  • image registration

Abstract

In this article, we present a novel method for the automatic 3D reconstruction of thick tissue blocks from 2D histological sections. The algorithm completes a high-content (multiscale, multifeature) imaging system for simultaneous morphological and molecular analysis of thick tissue samples. This computer-based system integrates image acquisition, annotation, registration, and three-dimensional reconstruction. We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high-resolution molecular data. Microsc. Res. Tech. 73:1019–1029, 2010. © 2010 Wiley-Liss, Inc.

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