A microfluidic positioning chamber for long-term live-cell imaging
Article first published online: 8 OCT 2010
Copyright © 2010 Wiley-Liss, Inc.
Microscopy Research and Technique
Volume 74, Issue 6, pages 496–501, June 2011
How to Cite
Hanson, L., Cui, L., Xie, C. and Cui, B. (2011), A microfluidic positioning chamber for long-term live-cell imaging. Microsc. Res. Tech., 74: 496–501. doi: 10.1002/jemt.20937
- Issue published online: 20 MAY 2011
- Article first published online: 8 OCT 2010
- Manuscript Accepted: 18 AUG 2010
- Manuscript Received: 11 FEB 2010
- National Institute of Health (NIH). Grant Number: NS057906
- Bio-X interdisciplinary initiatives program at Stanford, Searle Scholar award, and Packard Fellowship
- time-lapse imaging;
- live-cell imaging;
We report a microfluidic positioning chamber (MPC) that can rapidly and repeatedly relocate the same imaging area on a microscope stage. The “roof” of the microfluidic chamber was printed with serials of coordinate numbers that act as positioning marks for mammalian cells that grow attached to the “floor” of the microfluidic chamber. MPC cell culture chamber provided a simple solution for tracking the same cell or groups of cells over days or weeks. The positioning marks were used to register time-lapse images of the same imaging area to single-pixel accuracy. Using MPC cell culture chamber, we tracked the migration, division, and differentiation of individual PC12 cells for over a week using bright field and fluorescence imaging. Microsc. Res. Tech., 2010. © 2010 Wiley-Liss, Inc.