Morphological characterization of the progenitor blood cells in canine and feline umbilical cord
Version of Record online: 1 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Microscopy Research and Technique
Volume 75, Issue 6, pages 766–770, June 2012
How to Cite
Brólio, M. P., Vidane, A. S., Zomer, H. D., Wenceslau, C. V., OzóRio, J. J., Martins, D. S., Miglino, M. A. and AmbróSio, C. E. (2012), Morphological characterization of the progenitor blood cells in canine and feline umbilical cord. Microsc. Res. Tech., 75: 766–770. doi: 10.1002/jemt.21123
- Issue online: 21 MAY 2012
- Version of Record online: 1 DEC 2011
- Manuscript Accepted: 18 OCT 2011
- Manuscript Received: 20 SEP 2011
- cell morphology;
- umbilical cord blood;
- hematopoietic progenitor cells
The umbilical cord blood (UCB) is an important source of hematopoietic stem cells with great deal of interest in regenerative medicine. The UCB cells have been extensively studied as an alternative to the bone marrow transplants. The challenge is to define specific methods to purify and characterize these cells in different animal species. This study is aimed at morphological characterization of progenitor cells derived from UCB highlighting relevant differences with peripheral blood of adult in dog and cats. Therefore, blood was collected from 18 dogs and 5 cats' umbilical cords from fetus in various developmental stages. The mononuclear cells were separated using the gradient of density Histopaque-1077. Characterization of CD34+ cells was performed by flow cytometric analysis and transmission electron microscopy. Granulocytes (ancestry of the basophiles, eosinophiles, and neutrophiles) and agranulocytes (represented by immature lymphocytes) were identified. We showed for the first time the ultrastructural features of cat UCB cells. Microsc. Res. Tech. 75:766–770, 2012. © 2011 Wiley Periodicals, Inc.