Orientation independent retardation imaging using quantitative polarized phase microscopy
Article first published online: 31 MAY 2012
Copyright © 2012 Wiley Periodicals, Inc.
Microscopy Research and Technique
Volume 75, Issue 10, pages 1416–1419, October 2012
How to Cite
Dragomir, N.M. and Roberts, A. (2012), Orientation independent retardation imaging using quantitative polarized phase microscopy. Microsc. Res. Tech., 75: 1416–1419. doi: 10.1002/jemt.22082
- Issue published online: 21 SEP 2012
- Article first published online: 31 MAY 2012
- Manuscript Accepted: 5 MAY 2012
- Manuscript Received: 15 NOV 2011
- Australian Research Council's Discovery Projects scheme. Grant Number: DP 0663365
- phase imaging;
- polarized light microscopy (PLI);
- fiber optics imaging
Simultaneous optical phase and retardation measurement of a birefringent specimen is demonstrated independently of a priori knowledge of the optic axis orientation. The two-dimensional retardation distribution in both magnitude and angle of the fast axis orientation is uniquely determined from transverse phase images recorded with a bright field transmission microscope using light polarized at a minimum of three different polarization orientations. This approach opens a new possibility for stain-free phase and orientation-independent retardation characterization of samples using only one polarizer without needing other additional optical elements traditionally used in polarimetric measurements. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc.