A simple “hands-off” apparatus to inflate concealed soft parts of the genitalia of small insect specimens
Version of Record online: 7 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Microscopy Research and Technique
Volume 76, Issue 3, pages 258–262, March 2013
How to Cite
Hünefeld, F., Brehm, G. and Pohl, H. (2013), A simple “hands-off” apparatus to inflate concealed soft parts of the genitalia of small insect specimens. Microsc. Res. Tech., 76: 258–262. doi: 10.1002/jemt.22161
- Issue online: 26 FEB 2013
- Version of Record online: 7 JAN 2013
- Manuscript Accepted: 20 NOV 2012
- Manuscript Received: 28 AUG 2012
We describe a simple, effective and relatively cheap instrument for the inflation of soft parts of insect genitalia, particularly the vesica (endophallus) of small male lepidopteran specimens. It can also be applied in female genitalia and for the hyperextension of postabdominal segments of the telescoping type. A fine glass capillary is mounted on a self-constructed micromanipulator system with capillary holder, moveably placed aside a microscope. The micromanipulator is constructed from the x- and y-pinions of a discarded microscope table and the z-pinion of a discarded microscope. Preparation is carried out as usual on a microscopic slide. The inflatable soft parts are pushed out mechanically and then fully inflated and subsequently hardened by pumping absolute alcohol with a medical syringe via a flexible PVC tube, the capillary holder and the glass capillary into the phallus. With affordable effort, our “hands-off”apparatus might considerably ease preparation. Microsc. Res. Tech. 76:258–262, 2013. © 2013 Wiley Periodicals, Inc.