Chloride-ATPase dephosphorylation in Aplysia gut
Article first published online: 30 MAY 2002
Copyright © 2002 Wiley-Liss, Inc.
Journal of Experimental Zoology
Volume 293, Issue 1, pages 89–93, 15 June 2002
How to Cite
GERENCSER, G. A. and ZHANG, J. (2002), Chloride-ATPase dephosphorylation in Aplysia gut. J. Exp. Zool., 293: 89–93. doi: 10.1002/jez.10098
- Issue published online: 30 MAY 2002
- Article first published online: 30 MAY 2002
- Manuscript Accepted: 25 JAN 2002
- Manuscript Received: 1 SEP 2001
The present study was done primarily to compare cation-ATPase dephosphorylation kinetics with a Cl−-ATPase's dephosphorylation kinetics because of the paucity of information in this area. Utilizing a proteoliposomal preparation containing Cl−-ATPase from Aplysia gut, it was demonstrated that dephosphorylation of this P-type ATPase was absolutely dependent upon Cl−. Adenosine triphosphate (ATP) concentrations directly stimulated dephosphorylation of Cl−-ATPase in the presence of increasing concentrations of Cl−. It was also shown that the calculated rate constant for E1-P disintegration was 20/sec. This rate constant value approximated E1-P rate constant disintegration values for other electrogenic, uniport P-type ATPases. Therefore, it was concluded from these results that the Cl−-ATPase dephosphorylation kinetics did not differ greatly from cation-ATPase dephosphorylation kinetics. © 2002 Wiley-Liss, Inc.