Ethanol induced notochord and spinal cord duplications in the embryo of the zebrafish, Brachydanio rerio

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Abstract

Embryos of Brachydanio rerio, stage 10 (Hisaoka and Battle, '58) were exposed for 24 hours to graded concentrations of ethanol from 1 to 3%. Developmental abnormalities included defects of the eye, i.e., approximation, confluence and fusion of the optic cups mid-ventrally; as well as duplications of notochord and spinal cord. Duplication of the spinal cord in the hind brain region has not been reported previously. The duplicatory effects are discussed and a mechanism of duplication by infolding roof plate material is proposed.

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