Work supported by the U.S. Department of Energy.
Trophoblast regeneration by inner cell masses isolated from cultured mouse embryos†
Version of Record online: 25 APR 2005
Copyright © 1978 Wiley-Liss, Inc., A Wiley Company
Journal of Experimental Zoology
Volume 203, Issue 3, pages 483–489, March 1978
How to Cite
Spindle, A. I. (1978), Trophoblast regeneration by inner cell masses isolated from cultured mouse embryos. J. Exp. Zool., 203: 483–489. doi: 10.1002/jez.1402030315
- Issue online: 25 APR 2005
- Version of Record online: 25 APR 2005
The developmental potential of the inner cell mass (ICM) of the cultured mouse embryo was determined by testing the ability of the ICM to regenerate trophoblast in vitro. ICM's isolated by immunosurgery from either single or chimeric embryos were able to regenerate trophoblast when they were isolated at 69 hours of culture from the 2-cell stage, but they had lost this capacity by 93 hours of culture. Trophoblast regeneration by isolated ICM's did not appear to require either a critical cell mass at the time of isolation or cell proliferation during regeneration.