Supported by Grant PCM 76–11807 from the National Science Foundation.
Regenerative responses in larval axolotl limbs with skin grafts over the amputation surface†
Article first published online: 12 MAY 2005
Copyright © 1979 Wiley-Liss, Inc., A Wiley Company
Journal of Experimental Zoology
Volume 208, Issue 1, pages 97–109, April 1979
How to Cite
Tassava, R. A. and Garling, D. J. (1979), Regenerative responses in larval axolotl limbs with skin grafts over the amputation surface. J. Exp. Zool., 208: 97–109. doi: 10.1002/jez.1402080111
- Issue published online: 12 MAY 2005
- Article first published online: 12 MAY 2005
Complete flaps of whole skin over the amputation surface of larval axolotl limbs did not prevent dedifferentiation, DNA synthesis, and mitosis. However, even by two weeks, insufficient cell division had occurred for blastema formation. In about 50% of the cases, small areas of wound epidermis formed over the amputated tip of the radius or ulna. At two weeks after amputation, mitotic figures and dedifferentiated cells which incorporated H3-thymidine were predominantly located in the vicinity of the small area of wound epidermis. Although delayed in relation to controls, these limbs regenerated.
The results are consistent with the view that the wound epidermis is necessary to maintain dedifferentiated cells in the cell cycle.