Separation and labeling of specific subpopulations of Botryllus blood cells

Authors

  • Jay M. Schlumpberger,

    1. Laboratory of Experimental Oncology, Department of Pathology, Stanford University, Stanford, California 94305
    2. Hopkins Marine Station of Stanford University, Pacific Grove, California 93950
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  • Irving L. Weissman,

    1. Laboratory of Experimental Oncology, Department of Pathology, Stanford University, Stanford, California 94305
    2. Hopkins Marine Station of Stanford University, Pacific Grove, California 93950
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  • Dr. Virginia L. Scofield

    Corresponding author
    1. Laboratory of Experimental Oncology, Department of Pathology, Stanford University, Stanford, California 94305
    2. Hopkins Marine Station of Stanford University, Pacific Grove, California 93950
    • Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA 90024
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Abstract

Blood cells of the colonial tunicate Botryllus were separated by density gradient centrifugation in Percoll. Unseparated blood cells were used to immunize mice for development of hybridoma cell lines producing anti-Botryllus monoclonal antibodies. These antibodies identify specific subpopulations of blood cells, indicating possible divisions of these cells into defined subgroups sharing particular differentiation antigens. Additional studies utilizing fluorescein-conjugated lectins also revealed differential binding to density- and monoclonal antibody-defined blood cell fractions. These methods allow separation of the different Botryllus blood cell types for functional studies.

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