1932-5231/asset/olbannercenter.gif?v=1&s=3a5bfae8658d16961e7b8f5387220b6eb73c4e31)
Developmental Biology
Separation and labeling of specific subpopulations of Botryllus blood cells
Article first published online: 13 MAY 2005
DOI: 10.1002/jez.1402290308
Copyright © 1984 Wiley-Liss, Inc., A Wiley Company
1932-5231/asset/cover.gif?v=1&s=3153ec9d76a92ba5560b1bf33b1c7caaf5aa5be9 "Journal of Experimental Zoology")
Additional Information
How to Cite
Schlumpberger, J. M., Weissman, I. L. and Scofield, V. L. (1984), Separation and labeling of specific subpopulations of Botryllus blood cells. J. Exp. Zool., 229: 401–411. doi: 10.1002/jez.1402290308
Publication History
- Issue published online: 13 MAY 2005
- Article first published online: 13 MAY 2005
- Abstract
- References
- Cited By
Abstract
Blood cells of the colonial tunicate Botryllus were separated by density gradient centrifugation in Percoll. Unseparated blood cells were used to immunize mice for development of hybridoma cell lines producing anti-Botryllus monoclonal antibodies. These antibodies identify specific subpopulations of blood cells, indicating possible divisions of these cells into defined subgroups sharing particular differentiation antigens. Additional studies utilizing fluorescein-conjugated lectins also revealed differential binding to density- and monoclonal antibody-defined blood cell fractions. These methods allow separation of the different Botryllus blood cell types for functional studies.