Grant Sponsor: Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grants-in-Aid for Young Scientists); Grant number: 21677001; Grant Sponsor: JSPS Research Fellowships for Young Scientists.
Screening of Upregulated Genes Induced by High Density in the Vetch Aphid Megoura crassicauda
Article first published online: 18 APR 2012
© 2012 WILEY PERIODICALS, INC.
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume 317, Issue 3, pages 194–203, March 2012
How to Cite
How to cite this article: 2012. Screening of upregulated genes induced by high density in the vetch aphid megoura crassicauda. J. Exp. Zool. 317:194–203., , , , , , , .
Additional Supporting Information may be found in the online version of this article.
- Issue published online: 18 APR 2012
- Article first published online: 18 APR 2012
- Manuscript Accepted: 16 NOV 2011
- Manuscript Revised: 23 OCT 2011
- Manuscript Received: 27 JUN 2011
- Ministry of Education, Culture, Sports, Science, and Technology of Japan. Grant Number: 21677001
- JSPS Research Fellowships for Young Scientists
Aphids exhibit several polyphenisms in which discontinuous, alternative phenotypes are produced depending on environmental conditions. One representative example is the wing polyphenism, where winged and wingless females are produced through parthenogenesis. Previous work has shown that, in some aphid species, the density condition sensed by the mother aphid determines the developmental fate of embryos in her ovary, with high densities leading to winged progeny and low densities to wingless progeny. However, little is known about the molecular and physiological mechanisms underlying the wing polyphenism. To identify genes involved in the wing-morph determination in the vetch aphid, Megoura crassicauda, we compared maternal and embryonic transcripts between high- and low-density conditions using differential display, followed by quantitative real-time PCR (qRT-PCR). Under the high-density condition, two genes (Uba1 and Naca) were found to be upregulated in maternal tissues without ovaries, while one gene (ClpP) was upregulated in ovaries containing embryos. Uba1 and Naca encode factors that function in protein modification or transcriptional/translational regulation, respectively. In addition to differential display, candidate gene approaches focusing on morphogenetic and endocrine genes, i.e., wg, dpp, ap, hh, InR, IRS, Foxo, EcR, and USP, were also carried out. We found that wg was upregulated in maternal tissues under the high-density condition. The identified genes from both approaches are candidates for further study of their involvement in the transduction of density signals in mother aphids and/or the initial process of wing differentiation in embryos. J. Exp. Zool. 317:194–203, 2012. © 2012 Wiley Periodicals, Inc.