Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
Version of Record online: 30 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume 319, Issue 1, pages 39–52, January 2013
How to Cite
2012. Seasonal changes and regulation of the glucocorticoid receptor in the testis of the toad Rhinella arenarum. J. Exp. Zool.319A:39–52., , , , .
- Issue online: 18 DEC 2012
- Version of Record online: 30 NOV 2012
- Manuscript Accepted: 1 OCT 2012
- Manuscript Revised: 14 SEP 2012
- Manuscript Received: 9 AUG 2012
- Universidad de Buenos Aires. Grant Number: UBACYT X042
- Consejo Nacional de Investigaciones Científicas y Técnicas. Grant Number: PIP 2433
- Agencia Nacional de Promoción Científica y Tecnológica de la Argentina. Grant Number: PICT 32219
Several studies indicate that wild free-living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin-receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen-receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor-mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up-regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum. J. Exp. Zool. 319A:39–52, 2013. © 2012 Wiley Periodicals, Inc.