Cloning of Skeletal Myosin Heavy Chain Gene Family From Adult Pleopod Muscle and Whole Larvae of Shrimps
Article first published online: 21 MAR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume 319, Issue 5, pages 268–276, June 2013
How to Cite
2013. Cloning of skeletal myosin heavy chain gene family from adult pleopod muscle and whole larvae of shrimps. J. Exp. Zool. 319A:268–276., ,
- Issue published online: 10 MAY 2013
- Article first published online: 21 MAR 2013
- Manuscript Accepted: 14 FEB 2013
- Manuscript Revised: 11 FEB 2013
- Manuscript Received: 14 NOV 2012
- Japan Society for the Promotion of Science for Scientific Research (S). Grant Number: 19108003
The physiological and biological properties of skeletal muscle in crustacea have not been well understood compared with those of vertebrates. The present study focused on myosin, the major protein in skeletal muscle, from shrimps. In our previous study, two full-length genes encoding myosin heavy chain (MHC), a large subunit of the myosin molecule, were cloned from abdominal fast skeletal muscle of kuruma Marsupenaeus japonicus, black tiger Penaeus monodon and Pacific white Penaeus vannamei shrimps, and named as MHCa and MHCb. In this study, we renamed these as MHC1 and MHC2, respectively, due to the presence of various isoforms newly identified. Partial MHC sequences were identified from pleopod muscle of these shrimps. Two MHCs, named MHC3 and MHC4, were identified from pleopod muscle of kuruma shrimp, whereas two MHCs, named MHC4 and MHC5, were cloned from Pacific white shrimp pleopod. MHC3 was cloned only from black tiger shrimp pleopod. Partial MHC sequences from zoea, mysis, and postlarvae of black tiger and Pacific white shrimps were also determined. The phylogenetic tree demonstrated that most MHCs from pleopod muscle and larval MHCs formed clades with MHC1 and MHC2, respectively. These MHCs were considered to be of fast type, since MHC1 and MHC2 are fast-type MHCs according to our previous study. MHC5 obtained from pleopod muscle of Pacific white shrimp in this study was monophyletic with American lobster Homarus americanus S2 slow tonic MHC previously reported, indicating that MHC5 from Pacific white shrimp is of slow type. J. Exp. Zool. 319A:268–276, 2013. © 2013 © 2013 Wiley Periodicals, Inc.