Reproductive Biology

Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean daphnia†

  1. Christy D. Robinson1,
  2. Sebastian Lourido2,
  3. Sean P. Whelan3,
  4. Jeffry L. Dudycha4,
  5. Michael Lynch4,
  6. Sharon Isern5,*

Article first published online: 15 DEC 2005

DOI: 10.1002/jez.a.250

Issue

Journal of Experimental Zoology Part A: Comparative Experimental Biology

Journal of Experimental Zoology Part A: Comparative Experimental Biology

Volume 305A, Issue 1, pages 62–67, 1 January 2006

Additional Information

How to Cite

Robinson, C. D., Lourido, S., Whelan, S. P., Dudycha, J. L., Lynch, M. and Isern, S. (2006), Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean daphnia. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 305A: 62–67. doi: 10.1002/jez.a.250

Author Information

  1. 1

    Department Tropical Medicine, Tulane University, New Orleans, Louisiana 70112

  2. 2

    Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118

  3. 3

    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

  4. 4

    Department of Biology, Indiana University, Bloomington, Indiana 47405

  5. 5

    Center for Biomolecular Science and Engineering, Florida Gulf Coast University, Fort Myers, Florida 33965

*Center for Biomolecular Science and Engineering, Florida Gulf Coast University, College of Arts & Sciences, Whitaker Hall 250, 10501 FGCU Boulevard South, Fort Myers, FL 33965, USA

  1. †

    This publication is contribution #20011 for the Center for Biomolecular Science and Engineering, Florida Gulf Coast University.

Publication History

  1. Issue published online: 15 DEC 2005
  2. Article first published online: 15 DEC 2005
  3. Manuscript Accepted: 26 SEP 2005
  4. Manuscript Received: 15 JUN 2005

Funded by

  • US National Science Foundation. Grant Numbers: 0521917, 0328516
  • Coypu Foundation

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Abstract

The aim of this study was to determine whether a recombinant vesicular stomatitis virus (VSV) vector encoding a transgene could be used to infect and express a foreign gene in embryonic primary cell cultures derived from the freshwater microcrustacean Daphnia, the most widely used ecotoxicological model organism. To facilitate the evaluation of gene transfer, a reproducible method for establishing primary cultures from Daphnia embryonic tissues was developed. Within 24 hr after infection, transgene expression could be detected in cell culture. VSV was found to replicate in the cells with no apparent cytopathic effect. Here we report the first evidence of gene transfer and foreign gene expression in cultures of Daphnia embryonic cells using a recombinant viral vector. J. Exp. Zool. 305A:62–67, 2006. © 2005 Wiley-Liss, Inc.

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