Current address: Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, Hubei, P.R. China.
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Research Article
A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)
Article first published online: 17 MAY 2006
DOI: 10.1002/jez.b.21112
Copyright © 2006 Wiley-Liss, Inc., A Wiley Company
Issue
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 306B, Issue 6, pages 567–574, 15 November 2006
Additional Information
How to Cite
Luo, J., Lang, M., Salzburger, W., Siegel, N., Stölting, K. N. and Meyer, A. (2006), A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes). Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 306B: 567–574. doi: 10.1002/jez.b.21112
Publication History
- Issue published online: 20 NOV 2006
- Article first published online: 17 MAY 2006
- Manuscript Accepted: 30 MAR 2006
- Manuscript Received: 27 SEP 2005
Funded by
- Max-Planck Gesellschaft
- Universität Konstanz
- Deutsche Forschungsgemeinschaft
- Abstract
- References
- Cited By
Abstract
A goldfish (Carassius auratus auratus) bacterial artificial chromosome genomic library (BAC library) was constructed from one aquarium-bred male specimen (tetraploid, 4n=100, genome size=3.52 pg/cell). The library consists of 128,352 positive clones with an average insert size of 150.4 kb, covering the genome 11-fold. All clones were spotted onto nylon filters and thus are available for screening of genomic regions of interest, such as candidate genes, gene families, or large-sized syntenic DNA regions of cyprinid species. Preliminary screens with two genes were conducted with hybridizing probes to the genes RAG1 and lgi1. RAG1 is a single-copy gene in zebrafish and is duplicated in C. a. auratus. We found a very close correlation between the number of positive BAC clones and the expected library coverage. Two copies of lgi1 were found in zebrafish. We have detected four different copies in C. a. auratus, not in the expected abundance, which indicates some variation in the coverage of the BAC library. The preliminary screens indicate that many duplicated genes that resulted from the ancient fish-specific genome duplication persist in the tetraploid goldfish genome. Hence, the BAC library will provide a useful resource for the future work on comparative genomics, polyploidy, diploidization, and evolutionary genomics in fishes. J. Exp. Zool. (Mol. Dev. Eiol.) 306B, 2006. © 2006 Wiley-Liss, Inc.