Research Article

Pax6 expression during retinogenesis in sharks: comparison with markers of cell proliferation and neuronal differentiation†

  1. Susana Ferreiro-Galve,
  2. Isabel Rodríguez-Moldes,
  3. Eva Candal*

Article first published online: 3 NOV 2011

DOI: 10.1002/jez.b.21448

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Cover image for Vol. 318 Issue 3

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

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How to Cite

Ferreiro-Galve, S., Rodríguez-Moldes, I. and Candal, E. (2011), Pax6 expression during retinogenesis in sharks: comparison with markers of cell proliferation and neuronal differentiation. J. Exp. Zool.. doi: 10.1002/jez.b.21448

Author Information

  1. Department of Cell Biology and Ecology, University of Santiago de Compostela, Santiago de Compostela, Spain

*Department of Cell Biology and Ecology, CIBUS Bldg, University of Santiago de Compostela, 15782-Santiago de Compostela, Spain

  1. †
    ce

    ciliary epithelium

    CMZ

    ciliary marginal zone

    GCL

    ganglion cell layer

    HCL

    horizontal cell layer

    INL

    inner nuclear layer

    INLi

    inner part of the INL

    INLo

    outer part of the INL

    IPL

    inner plexiform layer

    Juv

    juvenile

    le

    lens epithelium

    nl

    nonlayered, outer part of the retina

    OF

    optic fissure

    ONH

    optic nerve head

    ONL

    outer nuclear layer

    OPL

    outer plexiform layer

    PH

    prehatching

    RPE

    retinal pigment epithelium

    TZ

    transition zone

Publication History

  1. Article first published online: 3 NOV 2011
  2. Manuscript Accepted: 13 SEP 2011
  3. Manuscript Revised: 3 AUG 2011
  4. Manuscript Received: 5 MAY 2011

Funded by

  • Spanish Dirección General de Investigación-FEDER. Grant Numbers: BFU2007-61154/BFI, BFU2010-15816
  • Xunta de Galicia. Grant Numbers: PGIDIT07PXIB200102PR, INCITE09ENA200048ES, 10PXIB200051PR

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Abstract

Pax6 is a highly conserved transcription factor that appears involved in the entire process of retinogenesis, including maintenance of proliferation of retinal progenitors and differentiation of particular neuron fates. To gain insight into the retinogenesis in fish, we study the dynamics of Pax6 expression in the developing and mature retina of two sharks that inhabit in particular environments, and compare it with the dynamics of a marker of cell proliferation (proliferating cell nuclear antigen, PCNA) and markers of neuronal differentiation, such as glutamic acid decarboxylase (GAD), calretinin (CR), tyrosine-hydroxylase, and serotonin (5-HT). Our results reveal that Pax6 is expressed in PCNA-immunoreactive cells within the nonlayered retina, suggesting a role for Pax6 in proliferating progenitors. Pax6 expression decays as development proceeds and eventually remains in some postmitotic cells, which points to additional roles of Pax6 following neurogenesis. Double immunofluorescence reveals Pax6/CR colocalization in the ganglion cell layer, Pax6/5-HT in the inner part of the inner nuclear layer (INLi), and Pax6/GAD in the INLi and horizontal cell layer. Our results suggest that Pax6 may contribute to neuron diversification in the neural retina. J. Exp. Zool. (Mol. Dev. Evol.) 314B, 2011. © 2011 Wiley Periodicals, Inc.

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