The authors declare no conflict of interest.
Dicer-Processed Small RNAs: Rules and Exceptions†
Article first published online: 16 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 320, Issue 1, pages 35–46, January 2013
How to Cite
2012. Dicer-Processed Small RNAs: Rules and Exceptions J. Exp. Zool. (Mol. Dev. Evol.)320B:35–46., , , .
- Issue published online: 27 DEC 2012
- Article first published online: 16 NOV 2012
- Manuscript Accepted: 13 SEP 2012
- Manuscript Revised: 15 JUL 2012
- Manuscript Received: 24 APR 2012
- Deutsche Forschungsgemeinschaft. Grant Numbers: SPP-1258, STA 850/7-2
- European Social Fund and the Free State of Saxony
Canonical microRNAs are excised from their hairpin-shaped precursors by Dicer. In order to find possible exceptions to this rule and to identify additional substrates for Dicer processing we re-evaluate the small RNA sequencing data of the Dicer knockdown experiment in MCF-7 cells orignally published by Friedländer et al. [Friedländer et al., 2012, Nucleic Acids Res 40:37–52]. While the well-known non-Dicer mir-451 is not sufficiently expressed in these experiments, there are several additional Dicer-independent microRNAs, among them the important tumor supressor mir-663a. We recover previously described examples of non-miRNA Dicer substrates such as tRNA-Gln and several snoRNAs. Interestingly, sdRNAs derived from box C/D snoRNAs are Dicer-independent, while those derived from box H/ACA snoRNAs are often Dicer dependent. Several pol-III transcripts, in particular the vault RNAs and the great ape specific snaRs are processed by Dicer, while the small RNAs originating from Y RNAs seem to be Dicer independent. J. Exp. Zool. (Mol. Dev. Evol.) 320B:35–46, 2013. © 2012 Wiley Periodicals, Inc.