Competing interests: The authors declare that they have no competing interests.
Mesoteloblast-Like Mesodermal Stem Cells in the Polychaete Annelid Platynereis dumerilii (Nereididae)†
Article first published online: 13 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 320, Issue 2, pages 94–104, March 2013
How to Cite
2013. Mesoteloblast-Like Mesodermal Stem Cells in the Polychaete Annelid Platynereis dumerilii (Nereididae) J. Exp. Zool. (Mol. Dev. Evol.) 320B:94–104., .
- Issue published online: 7 MAR 2013
- Article first published online: 13 FEB 2013
- Manuscript Accepted: 17 DEC 2012
- Manuscript Revised: 12 OCT 2012
- Manuscript Received: 5 APR 2012
- Marie Curie Research Training Network Zoonet. Grant Number: MRTN-CT-2004-005624
Spiral cleavage is observed in animals that belong to the lophotrochozoa, a large group of marine invertebrates. As characteristic for spiral cleavage, the bulk of mesoderm forms from one cell, the “4d blastomere.” This process has not yet been followed in cellular detail in annelids except in the leech, where “mesoteloblasts,” a pair of mesodermal stem cells, generate two bands of mesoderm precursor cells in an iterative fashion. It is so far unknown whether such stem cell-like lineage is a general property of 4d-derived mesoderm in spiralian larvae. To address this, we have analyzed the cell lineage of the 4d blastomere in the polychaete annelid Platynereis dumerilii, an emerging model for lophotrochozoan and spiralian embryology (Fischer et al., 2010), by 4D microscopy, a semi-automated cell tracking technique based on differential interference contrast serial imaging (Schnabel et al. '97). Our data reveal that the two daughter cells of the 4d cell undergo seven consecutive rounds of unequal cell divisions. They bud off smaller cells in ventral-vegetal orientation and thus show mesoteloblast- and stem cell-like behavior. Based on these findings, we suggest that mesoteloblast-like mesodermal stem cells that form continuous mesodermal bands are part of the Errantia + Sedentaria ground pattern. In the course of annelid evolution, the number consecutive divisions of these cells would have been low initially with <10 division cycles, giving rise to larval segments only, and then increased up to 35 as observed in clitellates. J. Exp. Zool. (Mol. Dev. Evol.) 320B:94–104, 2013. © 2013 Wiley Periodicals, Inc.