The Dynamics of Supernumerary Tooth Development Are Differentially Regulated by Sprouty Genes

Authors

  • SVATAVA LAGRONOVA-CHURAVA,

    Corresponding author
    1. Department of Teratology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
    2. Department of Anthropology and Human Genetics, Faculty of Science, Charles University, Prague, Czech Republic
    • Correspondence to: Svatava Churava, Department of Teratology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v. v. i., Videnska 1083, 14220 Prague, Czech Republic.

      E-mail: churava@biomed.cas.cz

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  • FRANTISEK SPOUTIL,

    1. Department of Teratology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
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  • SIMONA VOJTECHOVA,

    1. Department of Teratology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
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  • HERVE LESOT,

    1. INSERM UMR 1109, Team “Osteoarticular and Dental Regenerative NanoMedicine”, and Dental School, Université de Strasbourg, Strasbourg, France
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  • MIROSLAV PETERKA,

    1. Department of Teratology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
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  • OPHIR D. KLEIN,

    1. Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California San Francisco, San Francisco, California
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  • RENATA PETERKOVA

    1. Department of Teratology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
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ABSTRACT

In mice, a toothless diastema separates the single incisor from the three molars in each dental quadrant. In the prospective diastema of the embryo, small rudimentary buds are found that are presumed to be rudiments of suppressed teeth. A supernumerary tooth occurs in the diastema of adult mice carrying mutations in either Spry2 or Spry4. In the case of Spry2 mutants, the origin of the supernumerary tooth involves the revitalization of a rudimentary tooth bud (called R2), whereas its origin in the Spry4 mutants is not known. In addition to R2, another rudimentary primordium (called MS) arises more anteriorly in the prospective diastema. We investigated the participation of both rudiments (MS and R2) in supernumerary tooth development in Spry2 and Spry4 mutants by comparing morphogenesis, proliferation, apoptosis, size and Shh expression in the dental epithelium of MS and R2 rudiments. Increased proliferation and decreased apoptosis were found in MS and R2 at embryonic day (ED) 12.5 and 13.5 in Spry2−/− embryos. Apoptosis was also decreased in both rudiments in Spry4−/− embryos, but the proliferation was lower (similar to WT mice), and supernumerary tooth development was accelerated, exhibiting a cap stage by ED13.5. Compared to Spry2−/− mice, a high number of Spry4−/− supernumerary tooth primordia degenerated after ED13.5, resulting in a low percentage of supernumerary teeth in adults. We propose that Sprouty genes were implicated during evolution in reduction of the cheek teeth in Muridae, and their deletion can reveal ancestral stages of murine dental evolution. J. Exp. Zool. (Mol. Dev. Evol.) 320B:307–320, 2013. © 2013 Wiley Periodicals, Inc.

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