Antónia Monteiro, Bin Chen, Diane Ramos, and Jeffrey C. Oliver contributed equally to this work.
Distal-Less Regulates Eyespot Patterns and Melanization in Bicyclus Butterflies
Article first published online: 30 APR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 320, Issue 5, pages 321–331, July 2013
How to Cite
2013. Distal-less regulates eyespot patterns and melanization in Bicyclus butterflies. J. Exp. Zool. (Mol. Dev. Evol.) 320B:321–331., , , , , , , , .
Conflicts of interest: None.
Authors Contributions: See Acknowledgments for details.
- Issue published online: 10 JUN 2013
- Article first published online: 30 APR 2013
- Manuscript Accepted: 19 MAR 2013
- Manuscript Received: 9 MAR 2013
- NSF. Grant Numbers: IBN 0316283, IOB 0653399
Additional supporting information may be found in the online version of this article at the publisher's web-site.
|jezb22503-sm-0001-SupFig-S1.jpg||583K||Additional File 1. Schematic of vectors used in this study. All vectors are based on the transposable element piggyBac and include the 3xP3-EGFP marker cassette, and the heat-shock inducible promoter from Drosophila Hsp70 placed 5' of the inducible transgene. (A) Dll over-expression vector; (B) En-overexpression vector; (C) Sal-overexpression vector; (D) Dll-RNAi vector. The ampicillin resistance gene (amp) and plasmid origin of replication (ori), as well as the Drosophila white intron (in) are also included inside the piggyBac left and right arms in this last vector.|
|jezb22503-sm-0002-SupFig-S2.jpg||1178K||Additional File 2. Temporal dynamics of gene expression in Wild type and Spotty B. anynana forewings. Each row corresponds to one of eight wing compartments. In the M1 and Cu1 compartments, eyespots develop in both Wt and Spotty genotypes (solid rectangles); in the M2 and M3 compartments, eyespots only develop in the Spotty genotype (dashed rectangles).|
|jezb22503-sm-0003-SupFig-S3.jpg||319K||Additional File 3. Adult wild type and Spotty forewings. Nomenclature for the eight wing compartments analyzed in Additional file 2.|
|jezb22503-sm-0004-SupFig-S4.jpg||58K||Additional File 4. Number of transgenic and Wild type individuals used in the heat-shock experiments at different stages of development. Temp, temperature (in °C) the larvae were reared at; Stage, stage of development when the heat-shock was applied; Hs, heat-shocked animals; C, control animals; mal, males; fem, females.|
|jezb22503-sm-0005-SupFig-S5.jpg||1658K||Additional File 5. A 2 hr heat-shock raises protein levels in B. anynana larval forewings. Larval forewings of wild type (Wt) and Ultrabithorax (Ubx) over-expression transgenic lines are stained for Ubx protein expression (normally no protein is found in forewings) in control and heat-shocked individuals. (A) Wt control. (B) Wt wings following a heat-shock. (C) Ubx control. (D) Ubx wings following a heat-shock. (E) Ubx heat-shocked (hs) individuals have the brightest wings. Error bars are 95% CI of the mean brightness values. n = numbers of wings analyzed.|
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