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jezb22503-sup-0001-SupFig-S1.jpg583KAdditional File 1. Schematic of vectors used in this study. All vectors are based on the transposable element piggyBac and include the 3xP3-EGFP marker cassette, and the heat-shock inducible promoter from Drosophila Hsp70 placed 5' of the inducible transgene. (A) Dll over-expression vector; (B) En-overexpression vector; (C) Sal-overexpression vector; (D) Dll-RNAi vector. The ampicillin resistance gene (amp) and plasmid origin of replication (ori), as well as the Drosophila white intron (in) are also included inside the piggyBac left and right arms in this last vector.
jezb22503-sup-0002-SupFig-S2.jpg1178KAdditional File 2. Temporal dynamics of gene expression in Wild type and Spotty B. anynana forewings. Each row corresponds to one of eight wing compartments. In the M1 and Cu1 compartments, eyespots develop in both Wt and Spotty genotypes (solid rectangles); in the M2 and M3 compartments, eyespots only develop in the Spotty genotype (dashed rectangles).
jezb22503-sup-0003-SupFig-S3.jpg319KAdditional File 3. Adult wild type and Spotty forewings. Nomenclature for the eight wing compartments analyzed in Additional file 2.
jezb22503-sup-0004-SupFig-S4.jpg1260KAdditional File 4. Number of transgenic and Wild type individuals used in the heat-shock experiments at different stages of development. Temp, temperature (in °C) the larvae were reared at; Stage, stage of development when the heat-shock was applied; Hs, heat-shocked animals; C, control animals; mal, males; fem, females.
jezb22503-sup-0005-SupFig-S5.jpg1658KAdditional File 5. A 2 hr heat-shock raises protein levels in B. anynana larval forewings. Larval forewings of wild type (Wt) and Ultrabithorax (Ubx) over-expression transgenic lines are stained for Ubx protein expression (normally no protein is found in forewings) in control and heat-shocked individuals. (A) Wt control. (B) Wt wings following a heat-shock. (C) Ubx control. (D) Ubx wings following a heat-shock. (E) Ubx heat-shocked (hs) individuals have the brightest wings. Error bars are 95% CI of the mean brightness values. n = numbers of wings analyzed.

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