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Sequential Shh expression in the development of the mouse upper functional incisor

Authors

  • Maria Hovorakova,

    Corresponding author
    • Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Lucie Smrckova,

    1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    2. Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic
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  • Herve Lesot,

    1. INSERM UMR 1109, Team “Osteoarticular and Dental Regenerative NanoMedicine”, University of Strasbourg, Strasbourg, France
    2. Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France
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  • Katerina Lochovska,

    1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    2. Department of Anthropology and Human Genetics, Faculty of Science, Charles University, Prague, Czech Republic
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  • Miroslav Peterka,

    1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Renata Peterkova

    1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Conflicts of interest: None.

Correspondence to: Maria Hovorakova, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic.

E-mail: marhor@biomed.cas.cz

ABSTRACT

The mouse incisor is a frequently used model in studies of the molecular control of organ development. The appropriate interpretation of data on normogenesis is essential for understanding the data obtained in mutant mice. For this reason, we performed a very detailed investigation of the development of the upper incisor in wild-type mice from embryonic day (ED) 11.5 till 14.5. A combination of histology, whole mount in situ hybridization, computer-aided three-dimensional reconstructions, and fluorescent microscopy, has been used. Several sonic hedgehog (Shh) expression domains have been detected in the upper incisor region during early prenatal development. At ED11.5–13.5, there was a single Shh positive domain present in the anterior part of left or right upper jaw arches, corresponding to the epithelial thickening. More posteriorly, a new Shh expression domain appeared in the incisor bud in the developmentally more advanced ED13.5 embryos. At ED14.5, only this posterior Shh expression in the incisor germ remained detectable. This study brings new insights into the early development of the upper incisor in mice and completes the data on normal mouse incisor development. The temporal–spatial pattern of Shh expression reflects the development of two tooth generations, being detectable in two successive, antero-posteriorly located areas in the prospective incisor region in the upper jaw. The first, anterior and superficial Shh expression domain reflects the rudimentary tooth development suppressed during evolution. Only the subsequent, posterior and deeper Shh expression region, appearing at ED13.5, correlates with the prospective upper functional incisor in wild-type mice. J. Exp. Zool. (Mol. Dev. Evol.) 320B: 455–464, 2013. © 2013 Wiley Periodicals, Inc.

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