Transcriptional activity of transposable elements in coelacanth
Version of Record online: 3 SEP 2013
© 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Special Issue: Genome of the African Coelacanth
Volume 322, Issue 6, pages 379–389, September 2014
How to Cite
2013. Transcriptional activity of transposable elements in coelacanth. J. Exp. Zool. (Mol. Dev. Evol.) 322B:379–389., , , , , , , , , .
- Issue online: 6 AUG 2014
- Version of Record online: 3 SEP 2013
- Manuscript Accepted: 14 JUL 2013
- Manuscript Revised: 4 JUN 2013
- Manuscript Received: 29 MAR 2013
- Italian Ministry for University and Research Grant
- French Ministry for Higher Education and Research
The morphological stasis of coelacanths has long suggested a slow evolutionary rate. General genomic stasis might also imply a decrease of transposable elements activity. To evaluate the potential activity of transposable elements (TEs) in “living fossil” species, transcriptomic data of Latimeria chalumnae and its Indonesian congener Latimeria menadoensis were compared through the RNA-sequencing mapping procedures in three different organs (liver, testis, and muscle). The analysis of coelacanth transcriptomes highlights a significant percentage of transcribed TEs in both species. Major contributors are LINE retrotransposons, especially from the CR1 family. Furthermore, some particular elements such as a LF-SINE and a LINE2 sequences seem to be more expressed than other elements. The amount of TEs expressed in testis suggests possible transposition burst in incoming generations. Moreover, significant amount of TEs in liver and muscle transcriptomes were also observed. Analyses of elements displaying marked organ-specific expression gave us the opportunity to highlight exaptation cases, that is, the recruitment of TEs as new cellular genes, but also to identify a new Latimeria-specific family of Short Interspersed Nuclear Elements called CoeG-SINEs. Overall, transcriptome results do not seem to be in line with a slow-evolving genome with poor TE activity. J. Exp. Zool. (Mol. Dev. Evol.) 322B: 379–389, 2014. © 2013 Wiley Periodicals, Inc.