Anne Nitsche and Gero Doose are joint first authors.
Atypical RNAs in the coelacanth transcriptome
Article first published online: 30 OCT 2013
© 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Special Issue: Genome of the African Coelacanth
Volume 322, Issue 6, pages 342–351, September 2014
How to Cite
2013. Atypical RNAs in the coelacanth transcriptome. J. Exp. Zool. (Mol. Dev. Evol.) 322B:342–351., , , , , , , , , .
Conflicts of interest: None.
- Issue published online: 6 AUG 2014
- Article first published online: 30 OCT 2013
- Manuscript Accepted: 16 AUG 2013
- Manuscript Revised: 22 JUL 2013
- Manuscript Received: 24 APR 2013
- European Union FP-7 Project QUANTOMICS. Grant Number: 222664
- Federal Ministry of Education and Research in Germany (BMBF) Project ICGC MMML-Seq. Grant Number: 01KU1002J
- Italian Ministry for University and Research. Grant Numbers: UNIVPM6170, UNIVPM6823
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Comparison of splice junctions in normal (local and co-linear), circular, and trans-spliced reads. The ﬁrst three columns of the panel summarizes the distribution of distances of predicted splice junctions from the closest site with a canonical donor or acceptor motif (gt–ag). The remaining two columns show the distances to the nearest site that also harbours normally spliced reads.
Table S1. Split numbers.
Table S2. Summary of mapped reads.
Table S3. Comparison of observed splice junctions in L. chalumnae and L. menadoensis, resulting from reads mapped with local, colinear splits. The addition “filtered” describes the filtering by haarz mapping criteria and a minimum junction support of three reads.
Table S4. Relation of splice junctions to annotation.
Table S5. Conservation of normal splice sites.
Table S6. SHS-motives in circular and trans-spliced RNAs are indicative of “RTfacts.”
Table S7. Primers used to validate putative fused transcripts.
Table S8. Sequences for subcloned PCR products from validation experiment.
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