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Figure S1. Comparison of splice junctions in normal (local and co-linear), circular, and trans-spliced reads. The first three columns of the panel summarizes the distribution of distances of predicted splice junctions from the closest site with a canonical donor or acceptor motif (gtag). The remaining two columns show the distances to the nearest site that also harbours normally spliced reads.

Table S1. Split numbers.

Table S2. Summary of mapped reads.

Table S3. Comparison of observed splice junctions in L. chalumnae and L. menadoensis, resulting from reads mapped with local, colinear splits. The addition “filtered” describes the filtering by haarz mapping criteria and a minimum junction support of three reads.

Table S4. Relation of splice junctions to annotation.

Table S5. Conservation of normal splice sites.

Table S6. SHS-motives in circular and trans-spliced RNAs are indicative of “RTfacts.”

Table S7. Primers used to validate putative fused transcripts.

Table S8. Sequences for subcloned PCR products from validation experiment.

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