Trematode Himasthla elongata mariner element (Hemar): Structure and applications
Version of Record online: 16 DEC 2013
© 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 322, Issue 3, pages 142–155, May 2014
How to Cite
2014. Trematode Himasthla elongata mariner element (Hemar): Structure and applications. J. Exp. Zool. (Mol. Dev. Evol.) 322B:142–155., , , .
- Issue online: 8 APR 2014
- Version of Record online: 16 DEC 2013
- Manuscript Accepted: 19 NOV 2013
- Manuscript Received: 22 JUL 2013
- Russian Foundation for Basic Research. Grant Numbers: 02-04-49420-a, 05-04-49578-a, 09-04-01145-a
- RAS. Grant Number: N01200955639
- Ministry of Education and Science of Russia. Grant Number: N11.G34.31.0068
Additional Supporting Information may be found in the online version of this article at the publisher's web-site.
Figure S1. Identification of a 5′ and 3′ transposase flanking fragments. 5′ ITR—Partially unspecific amplification of H. elongata genomic DNA with HmOuR primer reveals the 1,070-bp sequence (arrow) containing 539 bp of Hemar1 5′ sequence. 3′ ITR—Inverse PCR followed by HindIII restriction reveals multiple bands. The band of 1,526 bp (arrow) contains 384 bp of Hemar1 sequence downstream from HmOuF.
Figure S2. Frame shifts inserted in the deduced amino acid sequences. The fixed oligopeptides are indicated. Each ORF is colored: 1st—red, 2nd—green, 3rd—blue. The shifts between ORF inserted in order to obtain best match without stop codons.
Figure S3. Comparison of Hemar fragments cloned. Nucleotide alignment of mariner element fragments (Hem1, Hem2, Hem3). 5′ and 3′ primers removed. MspI sites marked with arrowheads. Trinucleotides corresponding to the residues of D,D34D catalytic domain (Fig. 5, main text) indicated by the line above.
Figure S4. Relative quantification of Hemar1 transcripts in H. elongata cercarial genomes by the single step SYBR®Green real-time RT-PCR. Results from two different experiments are shown. mRNA was isolated from the mixture of H. elongata cercaria from different mollusks. −C: no template; +C: positive control; actin; primers to H. elongata actin gene; Hemar1: forward—specific Hem1 primer (HeC1_F, Table 1), reverse—degenerate (Mar276R, Table 1).
Table S1. p-distance of transposase core and Ka/Ks ratio of the corresponding nucleotides for the MLE from different species.
Table S2. p-distance of ITR and transposase of full-length MLE mostly close to Hemar1.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.