Sungdae Park and Carlos R. Infante contributed equally to this paper.
Conserved regulation of hoxc11 by pitx1 in Anolis lizards
Article first published online: 23 DEC 2013
© 2013 Wiley Periodicals, Inc.
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume 322, Issue 3, pages 156–165, May 2014
How to Cite
2014. Conserved regulation of hoxc11 by pitx1 in Anolis lizards. J. Exp. Zool. (Mol. Dev. Evol.) 322B:156–165., , , .
Conflicts of interest: None.
- Issue published online: 8 APR 2014
- Article first published online: 23 DEC 2013
- Manuscript Accepted: 26 NOV 2013
- Manuscript Received: 24 SEP 2013
- National Science Foundation. Grant Number: 1149453
- University of Georgia. Grant Number: 1021RX064211
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Efficient transfection of Anolis limb micromasses with the CMV promoter. (A) GFP positive cells are clearly visible in limb micromasses 48 hr after nucleofection. (B) Brightfield image of a subsample of dispersed limb micromass cells compared to (C) GFP fluorescence image of same cells reveals high efficiency of transfection.
Figure S2. Anolis hindlimb cells lose hindlimb-specific gene expression rapidly in culture. After the first passage of primary cell culture, the expression of both pitx1 and tbx4 is greatly decreased in Anolis hindlimb cells as measured by quantitative RT-PCR.
Figure S3. The expression of hoxc11 correlates with pitx1 expression in transfected Anolis forelimb micromasses and hindlimb controls. (A) Higher levels of ectopic pitx1 expression correlate with increased hoxc11 expression in transfected forelimb cells as measured by quantitative RT-PCR. (B) Non-nucleofected hindlimb micromass controls show a similar relationship between levels of pitx1 and hoxc11 expression.
Table S1. ΔCt values of assayed genes for pitx1-nucleofected treatment micromasses and GFP-nucleofected control micromasses and associated P-values for tests of significant differences of mean ΔCt values between treatments and controls.
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