Lentiviral small hairpin RNA delivery reduces apical sodium channel activity in differentiated human airway epithelial cells
Article first published online: 21 DEC 2012
Copyright © 2012 John Wiley & Sons, Ltd.
The Journal of Gene Medicine
Volume 14, Issue 12, pages 733–745, December 2012
How to Cite
Aarbiou, J., Copreni, E., Buijs-Offerman, R. M., van der Wegen, P., Castellani, S., Carbone, A., Tilesi, F., Fradiani, P., Hiemstra, P. S., Yueksekdag, G., Diana, A., Rosenecker, J., Ascenzioni, F., Conese, M. and Scholte, B. J. (2012), Lentiviral small hairpin RNA delivery reduces apical sodium channel activity in differentiated human airway epithelial cells. J. Gene Med., 14: 733–745. doi: 10.1002/jgm.2672
- Issue published online: 21 DEC 2012
- Article first published online: 21 DEC 2012
- Accepted manuscript online: 16 OCT 2012 11:01AM EST
- Manuscript Accepted: 7 OCT 2012
- Manuscript Revised: 27 SEP 2012
- Manuscript Received: 19 JUN 2012
The following supplementary material is available:
Doc. S1. Selection of shRNA sequence targeting ENaCα.
Figure S1. Relative expression of ENaCα mRNA in shRNAi-treated H441 cells, compared to mock transfected (Lipofectamine-treated) cells.
Figure S2. Effect of dexamethasone treatment on ENaCα mRNA expression and membrane potential.
Figure S3. Low concentration LPC enhances VSVG-LV transduction efficiency in differentiated HBEC-ALI cultures.
Table S1. Target sequence of the in silico selected shRNAs against the α ENaC gene.
Table S2. Primers used to study ENaC subunit expression by real time RT-PCR in CFBE cells.
This supplementary material can be found in the online article.
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