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Original Research Paper
Improved automated simultaneous determination of isosorbide dinitrate and its metabolites in plasma by capillary column gas chromatography
Article first published online: 10 APR 2005
DOI: 10.1002/jhrc.1240070202
Copyright © 1984 Dr. Alfred Huethig Publishers
1615-9314/asset/cover.gif?v=1&s=4399e530f19bbe52b672f645b0757dc884404878 "Journal of High Resolution Chromatography")
Additional Information
How to Cite
Lutz, D., Rasper, J., Gielsdorf, W., Settlage, J. A. and Jaeger, H. (1984), Improved automated simultaneous determination of isosorbide dinitrate and its metabolites in plasma by capillary column gas chromatography. J. High Resol. Chromatogr., 7: 58–65. doi: 10.1002/jhrc.1240070202
Publication History
- Issue published online: 10 APR 2005
- Article first published online: 10 APR 2005
- Manuscript Received: 13 MAY 1983
- Abstract
- References
- Cited By
Keywords:
- Gas chromatography/ECD;
- Capillary, fused silica;
- Isosorbide dinitrate;
- Isosorbide-/isomannide-/isoidide-mononitrates;
- Human plasma
Abstract
An automated specific and sensitive method for the simultaneous determination of isosorbide dinitrate and its metabolites isosorbide-2-mononitrate, isosorbide-5-mononitrate, isomannide mononitrate, and isoidide mononitrate at concentrations down to 0.5, 1, 3, 2, and 2 ng/ml, respectively, in human plasma is described. The procedure involves the extraction of the drug and its metabolites together with their internal standards isomannide dinitrate and o-nitrobenzyl alcohol from plasma with dicholromethane.
The analysis is carried out by fused silica capillary gas chromatography using a 63Ni-electron capture detector. The method is applied to the pharmacokinetics of isosorbide dinitrate in humans.