Capillary zone electrophoresis: Effect of physical parameters on separation efficiency and quantitation

Authors

  • K. D. Lukacs,

    1. Department of Chemistry, University of North Carolina Chapel Hill, North Carolina 27514, USA
    Search for more papers by this author
  • J. W. Jorgenson

    Corresponding author
    1. Department of Chemistry, University of North Carolina Chapel Hill, North Carolina 27514, USA
    • Department of Chemistry, University of North Carolina Chapel Hill, North Carolina 27514, USA
    Search for more papers by this author

Abstract

Separation efficiency in capillary zone electrophoresis was examined as a function of capillary length and diameter as well as solute concentration for a borosilicate glass capillary/phosphate buffer system. Electroosmotic flow coefficients in borosilicate, fused silica, and teflon capillaries were measured over the useful operating pH range of 3–8 at constant applied power (0.333 W). Sample introduction by a simple, low-dispersion electromigration method was found to be a reliable procedure for solute quantitation.

Ancillary