Paper published as part of a special issue on ‘Recent Developments in the Use of Isotopically Labelled Molecules in Chemistry and Biochemistry’.
Quantitative analysis with modern bioanalytical mass spectrometry and stable isotope labeling†
Article first published online: 9 AUG 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Journal of Labelled Compounds and Radiopharmaceuticals
Special Issue: Recent Developments on the Use of Isotopically Labelled Molecules in Chemistry and Biochemistry
Volume 50, Issue 11-12, pages 1124–1136, 15 - 30 October 2007
How to Cite
Richards, D. P., Sojo, L. E. and Keller, B. O. (2007), Quantitative analysis with modern bioanalytical mass spectrometry and stable isotope labeling. J Label Compd Radiopharm, 50: 1124–1136. doi: 10.1002/jlcr.1392
- Issue published online: 17 NOV 2007
- Article first published online: 9 AUG 2007
- Manuscript Accepted: 26 APR 2007
- Manuscript Received: 17 APR 2007
- UBC Child & Family Research Institute
- stable isotope labeling;
- mass spectrometry;
- protein quantitation;
- twin-ion technique
The invention of new ionization techniques namely electrospray ionization and matrix-assisted laser desorption/ionization combined with the development of novel mass spectrometer analyzers and evolving isotope-ratio mass spectrometry have fueled the presence and use of modern mass spectrometric methodologies in many bioanalytical laboratories. Consequently, over the past two decades, a steadily increasing number of quantitative methods employing stable isotope labeling techniques have been reported, including prominent examples of methods to determine differential expression of proteins in disease studies, new-born screening for metabolic disorders, and tracing drugs or dietary compounds and their respective metabolites. Labeling biomolecules for quantitative studies using mass spectrometry has several challenges, including potentially insufficient labeling efficiency, ionization suppression, chromatographic separation of labeled and non-labeled compounds, and isotope exchange with the environment. It is not surprising that method development to minimize or eliminate existing limitations represents a very active and dynamic research area. Copyright © 2007 John Wiley & Sons, Ltd.