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Biosynthesis of glucose-d-13C6 from Hansenula polymorpha and methanol-13C


Zhan-feng Liu, Shanghai Research Institute of Chemical Industry, Shanghai Engineering Research Center of Stable Isotope, No.345 Yunling East Road, 200062 Shanghai, China.



A simple and effective method for synthesis of glucose-d-13C6 by fermentation using the methylotrophic yeast Hansenula polymorpha with 99% abundance methanol-13C is described. Using methanol-13C as a sole source of carbon, H. polymorpha can accumulate large amounts of α,α-trehalose-13C12 under unfavourable growth conditions; the trehalose can then be hydrolysed to give glucose-d-13C6 with 98.5% abundance 13C. Copyright © 2011 John Wiley & Sons, Ltd.