Tritium labelling of PACAP-38 using a synthetic diiodinated precursor peptide


Martin Holst Friborg Pedersen, Hevesy Laboratory, Radiation Research Division, Risø-DTU, Technical University of Denmark, Frederiksborgvej 399, Dk-4000 Roskilde, Denmark.



In the interest of developing efficient methods for tritium labelling peptides, we here demonstrate the successful labelling of PACAP-38 (pituitary adenylate cyclase-activating polypeptide), a 38-mer peptide, using a synthetic diiodinated PACAP-38 precursor. In this example, we employ standard hydrogenation chemistry with the use of a heterogeneous palladium catalyst and carrier-free tritium gas on a tritium manifold system. Copyright © 2011 John Wiley & Sons, Ltd.